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ω-agatoxin IVA


Catalog N 11AGA001

Peptide properties

ω-agatoxin IVA is a peptide originally isolated from funnel web-spider venom Agelenopsis aperta. This peptide is a specific blocker of P/Q-type calcium channel (Cav2.1). It has been reported that ω-agatoxin IVA is a potent blocker of voltage-gated calcium channels in insect and vertebrate central neurons. The binding site for ω-agatoxin IVA has been localized in part to the extracellular S3–S4 loop in repeat IV of the α-1A Ca2+ channels, which is proximal to the S4 sensor domain. This is coherent with its functional effect (no pore-blocking activity, but gating modifier by a shift of channel activation towards more depolarized potentials). This makes this toxin a voltage-dependent blocker of P/Q calcium channels.

Product Specification

AA sequence: Lys-Lys-Lys-Cys4-Ile-Ala-Lys-Asp-Tyr-Gly-Arg-Cys12-Lys-Trp-Gly-Gly-Thr-Pro-Cys19-Cys20-Arg-Gly-Arg-Gly-Cys25-Ile-Cys27-Ser-Ile-Met-Gly-Thr-Asn-Cys34-Glu-Cys36-Lys-Pro-Arg-Leu-Ile-Met-Glu-Gly-Leu-Gly-Leu-Ala-OH
(Disulfide bonds between Cys4-Cys20, Cys12-Cys25, Cys19-Cys36 and Cys27-Cys34)
Length (aa): 48
Formula: C217H360N68O60S10
Molecular Weight: 5198.44 Da
Appearance: White lyophilized solid
Solubility: water and saline buffer
CAS number: 145017-83-0
Source: Synthetic
Purity rate: > 97 %

Price & quantities

  • 100 µg : 230 €
  • 500 µg : 750 €
  • 1 mg : 1275 €

Other quantities available upon request

Related products

 

ω-conotoxin MVIIA ω-conotoxin MVIIC ω-conotoxin GVIA ω-conotoxin SO3 SNX482

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References

  • Mintz I.M., et al. (1992) P-type calcium channels blocked by the spider toxin omega-Aga-IVA. Nature 355:827-829. PubMed link
  • Bourinet E, et al. (1999) Splicing of alpha 1A subunit gene generates phenotypic variants of P- and Q-type calcium channels. Nat Neurosci. May;2(5):407-15. PubMed link
  • Adams ME. (2004) Agatoxins: ion channel specific toxins from the American funnel web spider, Agelenopsis aperta.Toxicon Apr;43(5):509-25. PubMed link
  • Doering CJ, Zamponi GW. (2003) Molecular pharmacology of high voltage-activated calcium channels.J Bioenerg Biomembr. Dec; 35(6):491-505. PubMed link
  • Winterfield JR, Swartz KJ. (2000) A hot spot for the interaction of gating modifier toxins with voltage-dependent ion channels. J Gen Physiol. Nov;116(5):637-44. PubMed link
  • King GF. (2007) Modulation of insect Ca(v) channels by peptidic spider toxins. Toxicon. Mar 15;49(4):513-30. Epub 2006 Nov 28. PubMed link
  • Wicher D, Penzlin H. (1998) omega-Toxins affect Na+ currents in neurosecretory insect neurons. Receptors Channels. 5(6):355-66. PubMed link
  • Wicher D, Penzlin H. (1997) Ca2+ currents in central insect neurons: electrophysiological and pharmacological properties. J Neurophysiol. Jan;77(1):186-99. PubMed link

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