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Catalog N° 08GSM001

GsMTx4
Selective blocker of mechanosensitive ion channels

GsMTx4 (GsMTx-4, M-theraphotoxin-Gr1a) has been isolated from the venom of the spider Grammostola rosea. This cationic hydrophobic polypeptide blocks selectively the gating of cation selective channels and mechanosensitive ion channels such as TRPC1 or TRPC6, without having any effect on whole-cell voltage-sensitive currents. This toxin acts by perturbing the interface between the channel and the lipid bilayer without necessarily being in physical contact with the channel. GsMTx4 also demonstrated to active TRPA1 channel at 1µM concentration and to inhibit Piezo1 currents.

GsMTx4 technical information

AA sequence: Gly-Cys2-Leu-Glu-Phe-Trp-Trp-Lys-Cys9-Asn-Pro-Asn-Asp-Asp-Lys-Cys16-Cys17-Arg-Pro-Lys-Leu-Lys-Cys23-Ser-Lys-Leu-Phe-Lys-Leu-Cys30-Asn-Phe-Ser-Phe-NH2
Disulfide bonds: Cys2-Cys17, Cys9-Cys23 and Cys16-Cys30
Length (AA): 34
Formula: C185H273N49O45S6
Molecular Weight: 4095.2 Da
Appearance: White lyophilized solid
Solubility: water and saline buffer
CAS number:
Source: Synthetic
Purity rate: > 97 %

Price & Quantities


Reference # Product name Quantity (mg) Price (€) Ordering information
08GSM001-00050 GsMTx4 0.05 80
08GSM001-00100 GsMTx4 0.1 120
08GSM001-00500 GsMTx4 0.5 350

Other quantities available upon request



Special restriction: this product is not available for US customers

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Citation

  • Anderson M, et al. (2013) Opposing effects of podocin on the gating of podocyte TRPC6 channels evoked by membrane stretch or diacylglycerol. Am J Physiol Cell Physiol. PubMed link

References

  • Bae, C.,  et al. (2011) The Mechanosensitive Ion Channel Piezo1 Is Inhibited by the Peptide GsMTx4. Biochemistry. PubMed link
  • Kerstin Hill, Michael Schaefer (2007) TRPA1 Is Differentially Modulated by the Amphipathic Molecules Trinitrophenol and Chlorpromazine. JBC. PubMed link
  • Nishizawa M, Nishizawa K. (2007) Molecular dynamics simulations of a stretch-activated channel inhibitor GsMTx4 with lipid membranes: two binding modes and effects of lipid structure. Biophys J. PubMed link
  • Ruta, V., and MacKinnon, R. (2004) Localization of the voltage-sensor toxin receptor on KvAP, Biochemistry. PubMed link
  • Ostrow, K. L., et al. (2003) cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of mechanosensitive channels. Toxicon. PubMed link
  • Oswald, R. E., et al. (2002) Solution structure of peptide toxins that block mechanosensitive ion channels. J Biol Chem. PubMed link
  • Suchyna, T. M., et al. (2000) Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels. J Gen Physiol. PubMed link